Course in collaboration with the CUSO Molecular Plant Science (MPS) program.

Speaker: Laurent Mène-Saffrané, University of Fribourg

8-10 June 2020

The course has been canceled.

This course will provide the participants with the basic concepts on analytics and metabolomics. The course is theoretical and practical.

Mornings: 3 days x 3 hrs lecture "Introduction to analytics and metabolomics". The following topics will be approached:
- Principles of separation - Liquid and gas chromatography
- Principles of detection – Overview of 4 different detectors (DAD, FLD, MS, FID)
- Principles of quantification

Afternoons: 3 days x 4 hrs practical work with the analytical instruments:
- Fatty acid methyl ester analysis by gas chromatography/flame ionization detector
- Tocochromanol analysis by high-performance liquid chromatography/fluorescence
- Data analysis and reporting

Participants are invited to bring vegetable oil that they consumed (0.5 mL in an Eppendorf).
They will be analyzed during the practical part to illustrate the concepts developed during the three seminars.

Program

Day 1 - Monday 8 June
9h00 - 12h00: Seminar - Gas and Gas/liquid chromatography
13h30 - 17h30: Laboratory - HPLC analysis of tocochromanols

Day 2 - Tuesday 9 June
9h00 - 12h00: Seminar - Detectors
13h30 - 17h30: Laboratory - GC-FID analysis of fatty acid methyl esters

Day 3 - Wednesday 10 June
9h00 - 12h00: Seminar - Normalization, Quantification, PublicationDetectors
13h30 - 17h30: Laboratory - Calibration curves, detector responses, quantification

PROGRAM AND LOCATION

Date: 8-10 June 2020 (3 days) canceled

Schedule: 8.55-17.00

Venue:  University of Fribourg, Department of Biology, Rue Albert-Gockel, 3 (PER04 ; 0.111) MAP

ECTS: 1.5 (Research tools)

Evaluation: Full attendance and active participation

Information: Please contact Debora Zoia, coordinator of CUSO MPS, or Pauline Fritsch, coordinator of Neuchâtel doctoral program DPOB

Travel expenses: For participants of the Interuniversity doctoral program in organismal biology (DPOB ): see reimbursement conditions

For participants of the CUSO doctoral program in Molecular Plant Science, see MPS website

Make sure to sign the attendance sheet each and every day and to take your certificate of attendance at the end of the course (no certificate will be sent by mail)

• Maximum number of participants: 12
• Limited number of participants:

  • 12 places available for the Theoretical + Practical part:
    8 places for CUSO MPS student (already FULL) & 4 places for DPOB students

  • 8 places available for the Theoretical part ONLY (mornings):
    8 places available (as of 24.02.2020)

Registration through the web only: The course has been canceled.

Please note the cancellation policy (CHF 20).

Deadline: 8 May 2020